A Glove boxes have unique advantages in handling toxic and harmful substances, as they can provide a relatively safe environment for such operations. Firstly, the sealing performance of the glove box is good, which can effectively prevent toxic and harmful substances from leaking into the external environment. In terms of material selection, stainless steel is generally chosen as the material for the box, which has good corrosion resistance and chemical stability, and will not react with the toxic and harmful substances being processed, avoiding the generation of new dangers. Meanwhile, suitable gas environments can be selected based on the characteristics of different toxic and harmful substances. For example, for some easily oxidizable toxic substances, an inert gas atmosphere can be created to reduce the risk of oxidation reactions. However, when using glove boxes to handle toxic and harmful substances, operators still need to be highly cautious. We must strictly follow the operating procedures and have sufficient preparation and response measures for possible emergency situations, such as equipping emergency handling equipment, developing comprehensive emergency plans, etc., to ensure the safety of the entire handling process.