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FAQ

  • Q What is the role of glove boxes in antigen and adjuvant processing during vaccine preparation?

    A The glove box plays an important role in the antigen and adjuvant processing during vaccine preparation. Some protein antigens are prone to change their properties in aerobic or high humidity environments. The inert gas environment inside the glove box (such as nitrogen or argon) can effectively prevent antigen oxidation and denaturation, ensuring their activity. And when mixing adjuvants with antigens, the glove box can ensure a sterile environment during the mixing operation, greatly improving the quality of vaccines and providing a reliable and stable operating environment for antigen extraction, purification, and mixing with adjuvants, effectively promoting the smooth progress of vaccine preparation work.
  • Q What are the functions of glove boxes in vaccine research?

    A
    Glove boxes play a crucial role in sample processing and preservation in vaccine research.
    1. Glove boxes can prevent sample contamination. For large amounts of biological samples such as virus samples, cell samples, antigen samples, etc., their sealing structure and efficient filtration system can effectively block the entry of external microorganisms such as bacteria, fungi, etc., ensuring the purity of virus samples.
    2. Glove boxes can maintain the stability of samples, as mRNA components of mRNA vaccines are easily degraded by nucleases. The anaerobic and low humidity environment of glove boxes can reduce degradation and extend storage time, as glove boxes can accurately regulate internal temperature, humidity, and gas composition, providing stable storage conditions for vaccine components that are sensitive to the environment.
  • Q Can the glove box be wiped with alcohol?

    A It is generally not recommended to use alcohol to clean the inside of the glove box, as there may be other chemicals present in the glove box at the same time. Using alcohol to wipe may cause mixing with other chemicals, resulting in danger or affecting the experimental results; And excessive alcohol may cause an increase in humidity inside the glove box, while a low alcohol concentration may not achieve the expected cleaning and drying effects. The outer surface of the glove box can be wiped clean with alcohol, which has a certain volatility and can quickly evaporate without leaving any moisture outside the glove box. If unsure which cleaning agents can be used to clean the glove box, it is recommended to consult the glove box supplier.
  • Q What is the function and application field of the hundred level purification glove box?

    A The hundred level purification glove box provides a water free, oxygen free, and dust-free ultra pure working environment in experiments, which is crucial for experimental operations that require extremely high cleanliness. An integrated high-efficiency filter is used to draw in gas from the top of the FFU through a fan. After passing through the primary and high-efficiency filters, the clean gas is sent back to the box and combined with the purification system to form a closed cycle working system. The hundred level purification glove box can be applied in fields such as lithium-ion batteries and materials, semiconductors, laser welding, biomedicine, OLED, etc. These applications require extremely high cleanliness to ensure the accuracy of experiments and the high quality of products.
  • Q Are the requirements for the glove box transition compartment the same when transferring different items?

    A The transition compartment of the glove box has different operational requirements when transferring different items. For cotton and paper items, it is necessary to ensure their dryness and, if necessary, dry them in an oven before placing them in. For plastic bags and small items, they need to be handled properly to avoid blocking the transition chamber exhaust port. For bottles and cans, it is necessary to pass them through the opening to avoid excessive pressure difference between the inside of the bottle or can and the cabin, which may cause them to rupture. If bottles or cans cannot be opened, control the vacuum degree when vacuuming. These operational requirements are all aimed at ensuring that items can be safely and effectively transferred through the transition compartment to the glove box, while avoiding damage to the atmosphere inside the glove box.
  • Q What are the precautions when transferring items in the glove box transition compartment?

    A When transferring items in the transition compartment of the glove box, attention should be paid to three extractions and three restorations to avoid damaging the atmosphere inside the glove box, and to the dryness of the items. When placing the items in the transition compartment, avoid blocking the exhaust port; For bottles and cans, it is necessary to pass them through the opening or control the degree of vacuum appropriately. When the atmosphere inside the transition warehouse is uncertain, it is also necessary to make three draws and three supplements. In addition, it is important to avoid opening both the inner and outer compartments at the same time. Vacuum transition compartments are difficult to open, so do not attempt to forcefully open them. Do not open the inner compartment when there is external air inside the transition compartment. Regularly check the mechanical components and sealing rings of the glove box transition compartment to ensure its sealing.
  • Q Does the glove box vacuum pump need maintenance?

    A The glove box vacuum pump requires daily maintenance. By checking the oil level, oil quality, and paying attention to noise, the operating condition of the pump can be detected in a timely manner to ensure its normal operation. Appropriate oil level and clean pump oil help maintain good lubrication and sealing performance of the vacuum pump, avoiding a decrease in pumping efficiency due to poor lubrication or seal failure. Regular oil changes can remove impurities and pollutants from the oil, prevent corrosion or blockage of internal components of the pump, and extend the service life of the pump. If the glove box vacuum pump is not maintained regularly, insufficient oil level may cause the pump to lack sufficient lubrication during operation, resulting in wear and even damage to the pump body; Polluted pump oil will reduce the performance of the pump and affect the vacuum degree inside the box. Mikrouna kindly reminds that the maintenance of the glove box vacuum pump can be included in the daily inspection and maintenance of the glove box.
  • Q What are the daily maintenance aspects of glove box vacuum pump?

    A
    The daily maintenance of glove box vacuum pump covers various aspects.
    1. Check the oil level to ensure it is at 2/3, and add it promptly if it is insufficient;
    2. Check if the vacuum pump oil is contaminated, and replace it immediately if it is contaminated;
    3. Pay attention to whether there is any noise in the vacuum pump. If there is any noise, immediately contact the glove box supplier for technical support;
    4. Regularly clean the air inlet filter, which can prevent particulate matter from entering the pump chamber. The specific cleaning cycle depends on the usage situation.
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